Genotyping and primer details


Genotyping and primer details for 186A01 - At4g04450
Line ID 186A01
Locus At4g04450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2221082 of line 186A01 Chr4:2221082
   
Insertion allele amplicon 8474 - IS08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS08: 5'-AACCATATTACCGTGTTCCTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2220696
   
wt amplicon (over InsPos) 02rd - IS08
wt amplicon start Chr4:2220696
wt amplicon end Chr4:2221506
wt amplicon size 811 bp
Locus specific primer IS08: 5'-AACCATATTACCGTGTTCCTCAAG-3'
2nd locus specific primer 02rd: 5'-TACACAAACGGCTACACTGTAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11