Genotyping and primer details


Genotyping and primer details for 187B11 - At2g24550
Line ID 187B11
Locus At2g24550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10429194 of line 187B11 Chr2:10429194
   
Insertion allele amplicon 8474 - 11vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11vh: 5'-CCAAACCAAACCCAAACTTACTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10428708
   
wt amplicon (over InsPos) 26vu - 11vh
wt amplicon start Chr2:10428708
wt amplicon end Chr2:10429940
wt amplicon size 1233 bp
Locus specific primer 11vh: 5'-CCAAACCAAACCCAAACTTACTTA-3'
2nd locus specific primer 26vu: 5'-GCATTTTGAACGAGTCAAAGTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11