Genotyping and primer details


Genotyping and primer details for 188H04 - At4g27630
Line ID 188H04
Locus At4g27630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13793289 of line 188H04 Chr4:13793289
   
Insertion allele amplicon 8474 - 06wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06wu: 5'-TAAACATGATAATGCCAGAGCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13792695
   
wt amplicon (over InsPos) 27xd - 06wu
wt amplicon start Chr4:13792695
wt amplicon end Chr4:13793888
wt amplicon size 1194 bp
Locus specific primer 06wu: 5'-TAAACATGATAATGCCAGAGCAAG-3'
2nd locus specific primer 27xd: 5'-TTATTACCTGGTGGTCTTGTTACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11