Genotyping and primer details


Genotyping and primer details for 189E02 - At4g17860
Line ID 189E02
Locus At4g17860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9925854 of line 189E02 Chr4:9925854
   
Insertion allele amplicon 8474 - 12qv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12qv: 5'-CTATAAGCAACCTGCATTTCAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9926156
   
wt amplicon (over InsPos) 13qv - 12qv
wt amplicon start Chr4:9925445
wt amplicon end Chr4:9926156
wt amplicon size 711 bp
Locus specific primer 12qv: 5'-CTATAAGCAACCTGCATTTCAACA-3'
2nd locus specific primer 13qv: 5'-CTCGGTACAATCCTATATGCACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11