Genotyping and primer details


Genotyping and primer details for 194E01 - At1g66070
Line ID 194E01
Locus At1g66070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24596514 of line 194E01 Chr1:24596514
   
Insertion allele amplicon 8474 - 12uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12uv: 5'-TTCCAACTATATATTTCCGCAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24596041
   
wt amplicon (over InsPos) 42vl - 12uv
wt amplicon start Chr1:24596041
wt amplicon end Chr1:24596855
wt amplicon size 815 bp
Locus specific primer 12uv: 5'-TTCCAACTATATATTTCCGCAACC-3'
2nd locus specific primer 42vl: 5'-ACTTTGTGTATGGAAGAAGGCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11