Genotyping and primer details


Genotyping and primer details for 195B04 - At1g80210
Line ID 195B04
Locus At1g80210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30164788 of line 195B04 Chr1:30164788
   
Insertion allele amplicon 8474 - KR03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR03: 5'-TCCTGAGTTTCAAATCTTGTTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30165329
   
wt amplicon (over InsPos) 31rd - KR03
wt amplicon start Chr1:30164228
wt amplicon end Chr1:30165329
wt amplicon size 1102 bp
Locus specific primer KR03: 5'-TCCTGAGTTTCAAATCTTGTTGTG-3'
2nd locus specific primer 31rd: 5'-GTTCACAAAGAAAGCCTCAAAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11