Genotyping and primer details


Genotyping and primer details for 200A03 - At5g02500
Line ID 200A03
Locus At5g02500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:555809 of line 200A03 Chr5:555809
   
Insertion allele amplicon 8474 - DM18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DM18: 5'-GTAGCCTTTTTGTCAAGACCGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:555430
   
Insertion allele amplicon 8474 - DM19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DM19: 5'-TTTCCAAATCTTCCGATAAAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:556355
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11