Genotyping and primer details


Genotyping and primer details for 200C12 - At4g01170
Line ID 200C12
Locus At4g01170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:499746 of line 200C12 Chr4:499746
   
Insertion allele amplicon 8474 - 06fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06fm: 5'-CGGTGTACACTCTTCTACATCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:500435
   
wt amplicon (over InsPos) 02vu - 06fm
wt amplicon start Chr4:499072
wt amplicon end Chr4:500435
wt amplicon size 1364 bp
Locus specific primer 06fm: 5'-CGGTGTACACTCTTCTACATCCAA-3'
2nd locus specific primer 02vu: 5'-TTATATAGCCGTCGTAGCCACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11