Genotyping and primer details


Genotyping and primer details for 204G11 - At3g50110
Line ID 204G11
Locus At3g50110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18583620 of line 204G11 Chr3:18583620
   
Insertion allele amplicon 8474 - 05gg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05gg: 5'-TAAGGTGCCTCAGTTTCAAAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18583298
   
wt amplicon (over InsPos) 08hf - 05gg
wt amplicon start Chr3:18583298
wt amplicon end Chr3:18584129
wt amplicon size 832 bp
Locus specific primer 05gg: 5'-TAAGGTGCCTCAGTTTCAAAGAAT-3'
2nd locus specific primer 08hf: 5'-TTAGCCAAAACCTTTAGCTGGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11