Genotyping and primer details


Genotyping and primer details for 205C02 - At2g26560
Line ID 205C02
Locus At2g26560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11295389 of line 205C02 Chr2:11295389
   
Insertion allele amplicon 8474 - EO05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EO05: 5'-CTTGTTCTGTAAGATGCAAATGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11295721
   
wt amplicon (over InsPos) 21cu - EO05
wt amplicon start Chr2:11294905
wt amplicon end Chr2:11295721
wt amplicon size 817 bp
Locus specific primer EO05: 5'-CTTGTTCTGTAAGATGCAAATGGA-3'
2nd locus specific primer 21cu: 5'-TTGTATTCTTTAGCGTTTCCGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11