Genotyping and primer details


Genotyping and primer details for 205D04 - At3g24130
Line ID 205D04
Locus At3g24130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8711904 of line 205D04 Chr3:8711904
   
Insertion allele amplicon 8474 - BL93
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL93: 5'-CAAACAAAACGATGCACTACAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8711365
   
Insertion allele amplicon 8474 - BL94
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL94: 5'-GGCCAAGGAATTGTTAACAGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8712446
   
wt amplicon (over InsPos) 23ir - BL94
wt amplicon start Chr3:8711331
wt amplicon end Chr3:8712446
wt amplicon size 1116 bp
Locus specific primer BL94: 5'-GGCCAAGGAATTGTTAACAGTAGA-3'
2nd locus specific primer 23ir: 5'-GTGGTACGTAATTGGATAAAACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11