Genotyping and primer details


Genotyping and primer details for 207F09 - At1g06410
Line ID 207F09
Locus At1g06410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1957990 of line 207F09 Chr1:1957990
   
Insertion allele amplicon 8474 - 50wi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50wi: 5'-GTGTCTTTTTCTTAGGTGGTCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1957462
   
Insertion allele amplicon 3144 - 79wk
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 79wk: 5'-TTACATCCCCAACGATAAGAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1958772
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11