Genotyping and primer details


Genotyping and primer details for 208H02 - At4g08850
Line ID 208H02
Locus At4g08850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5638437 of line 208H02 Chr4:5638437
   
Insertion allele amplicon 8474 - 35wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35wp: 5'-TATAAGACAAAGCATGAGCCACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5637820
   
Insertion allele amplicon 8474 - JD12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JD12: 5'-GAGATTTGGAACATGACACAGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5639008
   
Insertion allele amplicon 8474 - MN50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN50: 5'-ATTCTCCTGTTGCTTTGATGATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5638174
   
wt amplicon (over InsPos) MN50 - JD12
wt amplicon start Chr4:5638174
wt amplicon end Chr4:5639008
wt amplicon size 835 bp
Locus specific primer JD12: 5'-GAGATTTGGAACATGACACAGCTA-3'
2nd locus specific primer MN50: 5'-ATTCTCCTGTTGCTTTGATGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11