Genotyping and primer details


Genotyping and primer details for 209A05 - At4g13500
Line ID 209A05
Locus At4g13500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7857268 of line 209A05 Chr4:7857268
   
Insertion allele amplicon 8474 - 35ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35ra: 5'-GGCTAGTGATTCCTCAACTTCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7857830
   
wt amplicon (over InsPos) 16rt - 35ra
wt amplicon start Chr4:7856720
wt amplicon end Chr4:7857830
wt amplicon size 1111 bp
Locus specific primer 35ra: 5'-GGCTAGTGATTCCTCAACTTCATC-3'
2nd locus specific primer 16rt: 5'-TGCCAACTAGGCTCTATGTCACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11