Genotyping and primer details


Genotyping and primer details for 209A08 - At1g16380
Line ID 209A08
Locus At1g16380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5599008 of line 209A08 Chr1:5599008
   
Insertion allele amplicon 8474 - UK04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer UK04: 5'-CTTCGTGTATCGATCGTGTTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5599306
   
wt amplicon (over InsPos) 13dd - UK04
wt amplicon start Chr1:5598516
wt amplicon end Chr1:5599306
wt amplicon size 791 bp
Locus specific primer UK04: 5'-CTTCGTGTATCGATCGTGTTTCTA-3'
2nd locus specific primer 13dd: 5'-TACATCCATATTTGATGAAGCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11