Genotyping and primer details


Genotyping and primer details for 210H03 - At1g66500
Line ID 210H03
Locus At1g66500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24811761 of line 210H03 Chr1:24811761
   
Insertion allele amplicon 8474 - 11us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11us: 5'-TCTAAAATTCTCCTCTCTCAACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24812619
   
wt amplicon (over InsPos) 33vw - 11us
wt amplicon start Chr1:24811123
wt amplicon end Chr1:24812619
wt amplicon size 1497 bp
Locus specific primer 11us: 5'-TCTAAAATTCTCCTCTCTCAACGC-3'
2nd locus specific primer 33vw: 5'-TGACACATAAGAGTAAAGAGGCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11