Genotyping and primer details


Genotyping and primer details for 210H07 - At1g10850
Line ID 210H07
Locus At1g10850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3613304 of line 210H07 Chr1:3613304
   
Insertion allele amplicon 8474 - 13sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13sa: 5'-AATAAGCTCTCTGGTCAAATTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3612819
   
wt amplicon (over InsPos) 33sq - 13sa
wt amplicon start Chr1:3612819
wt amplicon end Chr1:3613640
wt amplicon size 822 bp
Locus specific primer 13sa: 5'-AATAAGCTCTCTGGTCAAATTCCA-3'
2nd locus specific primer 33sq: 5'-ATGTATCCAAGTTTCGATTCAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11