Genotyping and primer details


Genotyping and primer details for 211D07 - At4g25360
Line ID 211D07
Locus At4g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12970009 of line 211D07 Chr4:12970009
   
Insertion allele amplicon 8474 - BL95
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL95: 5'-CCTGATTGAACATCAGTCTTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12970614
   
wt amplicon (over InsPos) 21ho - BL95
wt amplicon start Chr4:12969440
wt amplicon end Chr4:12970614
wt amplicon size 1175 bp
Locus specific primer BL95: 5'-CCTGATTGAACATCAGTCTTTTCA-3'
2nd locus specific primer 21ho: 5'-CGAAATATACGCAATGTTATGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11