Genotyping and primer details


Genotyping and primer details for 211F09 - At5g41140
Line ID 211F09
Locus At5g41140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16469617 of line 211F09 Chr5:16469617
   
Insertion allele amplicon 8474 - 17lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17lm: 5'-CGGAGGGATTAACAATCTCTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16469080
   
wt amplicon (over InsPos) 05mj - 17lm
wt amplicon start Chr5:16469080
wt amplicon end Chr5:16470029
wt amplicon size 950 bp
Locus specific primer 17lm: 5'-CGGAGGGATTAACAATCTCTGTAG-3'
2nd locus specific primer 05mj: 5'-ATCCAGTTCAGAGACACTGTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11