Genotyping and primer details


Genotyping and primer details for 212C11 - At2g19230
Line ID 212C11
Locus At2g19230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8345987 of line 212C11 Chr2:8345987
   
Insertion allele amplicon 8474 - JD11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JD11: 5'-TCTGATTCATGTCGAAACAAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8346312
   
Insertion allele amplicon 3144 - KC86
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KC86: 5'-CAAAAATTGACTACATGAGGCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8345666
   
wt amplicon (over InsPos) JD11 - KC86
wt amplicon start Chr2:8345666
wt amplicon end Chr2:8346312
wt amplicon size 647 bp
Locus specific primer KC86: 5'-CAAAAATTGACTACATGAGGCTGA-3'
2nd locus specific primer JD11: 5'-TCTGATTCATGTCGAAACAAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11