Genotyping and primer details


Genotyping and primer details for 212D11 - At3g06455
Line ID 212D11
Locus At3g06455 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1981765 of line 212D11 Chr3:1981765
   
Insertion allele amplicon 8474 - 06vi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06vi: 5'-AAGTTTAGGCTTGGTGACTGAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1982293
   
wt amplicon (over InsPos) 33vq - 06vi
wt amplicon start Chr3:1981140
wt amplicon end Chr3:1982293
wt amplicon size 1154 bp
Locus specific primer 06vi: 5'-AAGTTTAGGCTTGGTGACTGAAAA-3'
2nd locus specific primer 33vq: 5'-GGATCGTTACTTCGTAATGTGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11