Genotyping and primer details


Genotyping and primer details for 213A12 - At3g54950
Line ID 213A12
Locus At3g54950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20360085 of line 213A12 Chr3:20360085
   
Insertion allele amplicon 8474 - JK51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK51: 5'-CTTTGGCTTATCTAGAACACGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20360428
   
wt amplicon (over InsPos) 01bx - JK51
wt amplicon start Chr3:20359585
wt amplicon end Chr3:20360428
wt amplicon size 844 bp
Locus specific primer JK51: 5'-CTTTGGCTTATCTAGAACACGCTC-3'
2nd locus specific primer 01bx: 5'-TGAAGTTATCAAATGCAATTCTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11