Genotyping and primer details


Genotyping and primer details for 213B12 - At1g76110
Line ID 213B12
Locus At1g76110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28557157 of line 213B12 Chr1:28557157
   
Insertion allele amplicon 3144 - 01bd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01bd: 5'-CCGTTACTTTTAAATCCGTAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28557670
   
Insertion allele amplicon 8474 - 13aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13aq: 5'-TTTGTAGTAAGAATGTGTCCCACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28556528
   
wt amplicon (over InsPos) 01bd - 13aq
wt amplicon start Chr1:28556528
wt amplicon end Chr1:28557670
wt amplicon size 1143 bp
Locus specific primer 13aq: 5'-TTTGTAGTAAGAATGTGTCCCACG-3'
2nd locus specific primer 01bd: 5'-CCGTTACTTTTAAATCCGTAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11