Genotyping and primer details


Genotyping and primer details for 214B03 - At1g20380
Line ID 214B03
Locus At1g20380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7062183 of line 214B03 Chr1:7062183
   
Insertion allele amplicon 8474 - 28by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28by: 5'-AGCTGGATAGCGACTGTAGAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7062782
   
wt amplicon (over InsPos) 02dk - 28by
wt amplicon start Chr1:7061772
wt amplicon end Chr1:7062782
wt amplicon size 1011 bp
Locus specific primer 28by: 5'-AGCTGGATAGCGACTGTAGAAAAA-3'
2nd locus specific primer 02dk: 5'-CTGGTAAAAAGCGTTTACGTTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11