Genotyping and primer details


Genotyping and primer details for 215D11 - At1g29030
Line ID 215D11
Locus At1g29030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10132027 of line 215D11 Chr1:10132027
   
Insertion allele amplicon 8474 - CS28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS28: 5'-ATAAGCTTTTTGTTGAGAGGGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10131557
   
wt amplicon (over InsPos) 13si - CS28
wt amplicon start Chr1:10131557
wt amplicon end Chr1:10132308
wt amplicon size 752 bp
Locus specific primer CS28: 5'-ATAAGCTTTTTGTTGAGAGGGTGA-3'
2nd locus specific primer 13si: 5'-AGTAGGTACTGCCATACTGGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11