Genotyping and primer details


Genotyping and primer details for 216D07 - At5g23670
Line ID 216D07
Locus At5g23670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7983815 of line 216D07 Chr5:7983815
   
Insertion allele amplicon 8474 - MC01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MC01: 5'-ATCGACCATGGAAGAAAATTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7983305
   
Insertion allele amplicon 8474 - MC02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MC02: 5'-TCATGACCTGATTAGATTGTGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7984337
   
wt amplicon (over InsPos) MC02 - MC01
wt amplicon start Chr5:7983305
wt amplicon end Chr5:7984337
wt amplicon size 1032 bp
Locus specific primer MC01: 5'-ATCGACCATGGAAGAAAATTATTG-3'
2nd locus specific primer MC02: 5'-TCATGACCTGATTAGATTGTGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11