Genotyping and primer details


Genotyping and primer details for 216D09 - At4g21160
Line ID 216D09
Locus At4g21160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11285129 of line 216D09 Chr4:11285129
   
Insertion allele amplicon 8474 - 03im
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03im: 5'-GACTGATTTATCCACCTTGTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11284789
   
wt amplicon (over InsPos) 05gz - 03im
wt amplicon start Chr4:11284789
wt amplicon end Chr4:11285672
wt amplicon size 883 bp
Locus specific primer 03im: 5'-GACTGATTTATCCACCTTGTTGGT-3'
2nd locus specific primer 05gz: 5'-AGTGCGAAAGCTATCAACAATCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11