Genotyping and primer details


Genotyping and primer details for 218D01 - At1g68400
Line ID 218D01
Locus At1g68400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25648204 of line 218D01 Chr1:25648204
   
Insertion allele amplicon 3144 - MN56
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MN56: 5'-GAATCCTCCTTTCCCTAACATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25647798
   
Insertion allele amplicon 8474 - PP19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP19: 5'-CCTCACTCACATCACTCAGAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25648654
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11