Genotyping and primer details


Genotyping and primer details for 222A08 - At5g43745
Line ID 222A08
Locus At5g43745 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17571954 of line 222A08 Chr5:17571954
   
Insertion allele amplicon 8474 - 06kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06kn: 5'-TGACATGCTAGCTATTAAGCGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17572381
   
wt amplicon (over InsPos) 06le - 06kn
wt amplicon start Chr5:17571544
wt amplicon end Chr5:17572381
wt amplicon size 838 bp
Locus specific primer 06kn: 5'-TGACATGCTAGCTATTAAGCGAAA-3'
2nd locus specific primer 06le: 5'-ATAAGGTCTTTTTGACGTGAGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11