Genotyping and primer details


Genotyping and primer details for 222C01 - At4g18100
Line ID 222C01
Locus At4g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10036216 of line 222C01 Chr4:10036216
   
Insertion allele amplicon 8474 - 06wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06wm: 5'-AGGCCGTGAAAGAAATCTTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10036628
   
wt amplicon (over InsPos) 15xd - 06wm
wt amplicon start Chr4:10034954
wt amplicon end Chr4:10036628
wt amplicon size 1675 bp
Locus specific primer 06wm: 5'-AGGCCGTGAAAGAAATCTTATTG-3'
2nd locus specific primer 15xd: 5'-TAAACTCCAAATTTACGCGTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11