Genotyping and primer details


Genotyping and primer details for 222C12 - At4g33180
Line ID 222C12
Locus At4g33180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16000893 of line 222C12 Chr4:16000893
   
Insertion allele amplicon 8474 - 05do
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05do: 5'-AGAGCATCAGGAAACATACGAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16001419
   
Insertion allele amplicon 8474 - 08eb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08eb: 5'-TTGGATCTTTGCAATAAACTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16001311
   
wt amplicon (over InsPos) 08vu - 05do
wt amplicon start Chr4:16000274
wt amplicon end Chr4:16001419
wt amplicon size 1146 bp
Locus specific primer 05do: 5'-AGAGCATCAGGAAACATACGAACT-3'
2nd locus specific primer 08vu: 5'-AGAAAGATATGTCGCTGTCGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11