Genotyping and primer details


Genotyping and primer details for 222D01 - At1g17100
Line ID 222D01
Locus At1g17100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5844995 of line 222D01 Chr1:5844995
   
Insertion allele amplicon 3144 - 32au
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 32au: 5'-ACCACACATTCACACGTAACAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5844676
   
Insertion allele amplicon 8474 - KA26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA26: 5'-GAAGGGTGAGTTATACTGAGCGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5845485
   
wt amplicon (over InsPos) 32au - KA26
wt amplicon start Chr1:5844676
wt amplicon end Chr1:5845485
wt amplicon size 810 bp
Locus specific primer KA26: 5'-GAAGGGTGAGTTATACTGAGCGAC-3'
2nd locus specific primer 32au: 5'-ACCACACATTCACACGTAACAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11