Genotyping and primer details


Genotyping and primer details for 223D10 - At1g12850
Line ID 223D10
Locus At1g12850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4380916 of line 223D10 Chr1:4380916
   
Insertion allele amplicon 8474 - BJ27
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BJ27: 5'-AGCGAAGGTGAAGAAGTAGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4381514
   
wt amplicon (over InsPos) 08qy - BJ27
wt amplicon start Chr1:4380332
wt amplicon end Chr1:4381514
wt amplicon size 1183 bp
Locus specific primer BJ27: 5'-AGCGAAGGTGAAGAAGTAGAAGAA-3'
2nd locus specific primer 08qy: 5'-CTCTTCCCAGATTTCTTCCTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11