Genotyping and primer details


Genotyping and primer details for 229D04 - At4g16760
Line ID 229D04
Locus At4g16760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9425159 of line 229D04 Chr4:9425159
   
Insertion allele amplicon 8474 - 14hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14hw: 5'-GATTCTCCCTCTCAATGTCAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9425459
   
wt amplicon (over InsPos) 05ji - 14hw
wt amplicon start Chr4:9424725
wt amplicon end Chr4:9425459
wt amplicon size 735 bp
Locus specific primer 14hw: 5'-GATTCTCCCTCTCAATGTCAGTTC-3'
2nd locus specific primer 05ji: 5'-TGTTGCATTGAAAACGAAAAATAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11