Genotyping and primer details


Genotyping and primer details for 230H01 - At5g06530
Line ID 230H01
Locus At5g06530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1992917 of line 230H01 Chr5:1992917
   
Insertion allele amplicon 8474 - 20sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20sm: 5'-TCCACAGATGAAGTCAATTTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1992390
   
wt amplicon (over InsPos) 04ul - 20sm
wt amplicon start Chr5:1992390
wt amplicon end Chr5:1993383
wt amplicon size 994 bp
Locus specific primer 20sm: 5'-TCCACAGATGAAGTCAATTTCTTG-3'
2nd locus specific primer 04ul: 5'-ATTTCGCCTTCACTTAGTGTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11