Genotyping and primer details


Genotyping and primer details for 230H02 - At2g32340
Line ID 230H02
Locus At2g32340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13733369 of line 230H02 Chr2:13733369
   
Insertion allele amplicon 8474 - 04rl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04rl: 5'-AGAGGAAGTGGTCGATTAATCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13733896
   
wt amplicon (over InsPos) 03rt - 04rl
wt amplicon start Chr2:13733106
wt amplicon end Chr2:13733896
wt amplicon size 791 bp
Locus specific primer 04rl: 5'-AGAGGAAGTGGTCGATTAATCTTG-3'
2nd locus specific primer 03rt: 5'-TTTGTTTATTCTTGAAAGGTGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11