Genotyping and primer details


Genotyping and primer details for 233E11 - At2g46810
Line ID 233E11
Locus At2g46810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19240873 of line 233E11 Chr2:19240873
   
Insertion allele amplicon 8474 - NH25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH25: 5'-TAGTGCTAGAAAGCTTGGGTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19241377
   
Insertion allele amplicon 8474 - PH66
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH66: 5'-GTAGTGGTTGGTTTCAGAATGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19241479
   
wt amplicon (over InsPos) 31sq - PH66
wt amplicon start Chr2:19240309
wt amplicon end Chr2:19241479
wt amplicon size 1171 bp
Locus specific primer PH66: 5'-GTAGTGGTTGGTTTCAGAATGGAT-3'
2nd locus specific primer 31sq: 5'-ATTGGGTTGTAACAAAAATCAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11