Genotyping and primer details


Genotyping and primer details for 233F04 - At1g14560
Line ID 233F04
Locus At1g14560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4982201 of line 233F04 Chr1:4982201
   
Insertion allele amplicon 8474 - 12cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12cv: 5'-GGTGTTACAATTTGATGGTCCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4981665
   
wt amplicon (over InsPos) 30dy - 12cv
wt amplicon start Chr1:4981665
wt amplicon end Chr1:4982629
wt amplicon size 965 bp
Locus specific primer 12cv: 5'-GGTGTTACAATTTGATGGTCCTCT-3'
2nd locus specific primer 30dy: 5'-CAAATAAACCAGCTAAAGCTCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11