Genotyping and primer details


Genotyping and primer details for 236B06 - At3g21390
Line ID 236B06
Locus At3g21390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7534330 of line 236B06 Chr3:7534330
   
Insertion allele amplicon 8474 - 07qw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07qw: 5'-TTCTAGCTTTCAGCTTTTCCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7533969
   
wt amplicon (over InsPos) 10tx - 07qw
wt amplicon start Chr3:7533969
wt amplicon end Chr3:7534752
wt amplicon size 784 bp
Locus specific primer 07qw: 5'-TTCTAGCTTTCAGCTTTTCCTTTG-3'
2nd locus specific primer 10tx: 5'-TTTTAGAAAGTTTTGTGCCTCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11