Genotyping and primer details


Genotyping and primer details for 245A02 - At4g17200
Line ID 245A02
Locus At4g17200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9651662 of line 245A02 Chr4:9651662
   
Insertion allele amplicon 8474 - CP86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP86: 5'-TGTATTGCAGAGTTTCAAAGGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9651087
   
Insertion allele amplicon 8474 - CP87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP87: 5'-TCTTGTCCAATGATGTAAGCTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9652135
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11