Genotyping and primer details


Genotyping and primer details for 245D04 - At3g05010
Line ID 245D04
Locus At3g05010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1389888 of line 245D04 Chr3:1389888
   
Insertion allele amplicon 8474 - 04ey
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ey: 5'-TAATATCTTTTTGGTGGGCAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1389059
   
wt amplicon (over InsPos) 03gc - 04ey
wt amplicon start Chr3:1389059
wt amplicon end Chr3:1389962
wt amplicon size 904 bp
Locus specific primer 04ey: 5'-TAATATCTTTTTGGTGGGCAAAAG-3'
2nd locus specific primer 03gc: 5'-TGATCTCTTTACACGCAACATAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11