Genotyping and primer details


Genotyping and primer details for 247C03 - At1g70140
Line ID 247C03
Locus At1g70140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26415070 of line 247C03 Chr1:26415070
   
Insertion allele amplicon 8474 - 08di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08di: 5'-CAAGACCATTCTCATCAAGAATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26414574
   
Insertion allele amplicon 8474 - 83wr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 83wr: 5'-AAACCATGCCTTTTTGTTTTACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26415986
   
wt amplicon (over InsPos) 32dy - 08di
wt amplicon start Chr1:26414574
wt amplicon end Chr1:26415499
wt amplicon size 926 bp
Locus specific primer 08di: 5'-CAAGACCATTCTCATCAAGAATCA-3'
2nd locus specific primer 32dy: 5'-TTAGACTTCCAAAGCGTCAAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11