Genotyping and primer details


Genotyping and primer details for 250D06 - At2g47250
Line ID 250D06
Locus At2g47250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19400593 of line 250D06 Chr2:19400593
   
Insertion allele amplicon 8474 - 04ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ht: 5'-AGTGCGTATGAAATTCCGAGTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19400157
   
wt amplicon (over InsPos) 10io - 04ht
wt amplicon start Chr2:19400157
wt amplicon end Chr2:19401192
wt amplicon size 1036 bp
Locus specific primer 04ht: 5'-AGTGCGTATGAAATTCCGAGTAGT-3'
2nd locus specific primer 10io: 5'-ATAGATGTCGGTTTACTTGAGCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11