Genotyping and primer details


Genotyping and primer details for 252F11 - At1g28050
Line ID 252F11
Locus At1g28050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9776466 of line 252F11 Chr1:9776466
   
Insertion allele amplicon 8474 - 09nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09nt: 5'-CAATATGCTCTGTGAAATGCAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9776099
   
wt amplicon (over InsPos) 24oj - 09nt
wt amplicon start Chr1:9776099
wt amplicon end Chr1:9776974
wt amplicon size 876 bp
Locus specific primer 09nt: 5'-CAATATGCTCTGTGAAATGCAAGT-3'
2nd locus specific primer 24oj: 5'-ATGGTCAAGAGATGTTGAGGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11