Genotyping and primer details


Genotyping and primer details for 255B08 - At3g61670
Line ID 255B08
Locus At3g61670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22820927 of line 255B08 Chr3:22820927
   
Insertion allele amplicon 8474 - CK52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK52: 5'-CAGGAACATATTGACAAGAAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22821469
   
wt amplicon (over InsPos) 30ix - CK52
wt amplicon start Chr3:22820401
wt amplicon end Chr3:22821469
wt amplicon size 1069 bp
Locus specific primer CK52: 5'-CAGGAACATATTGACAAGAAGCAA-3'
2nd locus specific primer 30ix: 5'-ATGGAAGATGGCCTAGTAACTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11