Genotyping and primer details


Genotyping and primer details for 255G01 - At3g59820
Line ID 255G01
Locus At3g59820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22099230 of line 255G01 Chr3:22099230
   
Insertion allele amplicon 8474 - 21dh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21dh: 5'-GGTTTTGTATCAGACCATCAACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22098734
   
wt amplicon (over InsPos) 04ec - 21dh
wt amplicon start Chr3:22098734
wt amplicon end Chr3:22099663
wt amplicon size 930 bp
Locus specific primer 21dh: 5'-GGTTTTGTATCAGACCATCAACCT-3'
2nd locus specific primer 04ec: 5'-AGAGTTAAAGCAGAGGATGCTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11