Genotyping and primer details


Genotyping and primer details for 255G07 - At2g39980
Line ID 255G07
Locus At2g39980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16689693 of line 255G07 Chr2:16689693
   
Insertion allele amplicon 8474 - MZ32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ32: 5'-TTCTTCTTGTTCACCACAGCTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16689155
   
wt amplicon (over InsPos) 09si - MZ32
wt amplicon start Chr2:16689155
wt amplicon end Chr2:16690123
wt amplicon size 969 bp
Locus specific primer MZ32: 5'-TTCTTCTTGTTCACCACAGCTTTA-3'
2nd locus specific primer 09si: 5'-GGAAACACTTTTCCAAAGTACAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11