Genotyping and primer details


Genotyping and primer details for 256H06 - At5g48370
Line ID 256H06
Locus At5g48370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19598936 of line 256H06 Chr5:19598936
   
Insertion allele amplicon 8474 - 17uw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17uw: 5'-TAATGCATAGAGCATTTGAGTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19599321
   
wt amplicon (over InsPos) 69wa - 17uw
wt amplicon start Chr5:19598408
wt amplicon end Chr5:19599321
wt amplicon size 914 bp
Locus specific primer 17uw: 5'-TAATGCATAGAGCATTTGAGTTGG-3'
2nd locus specific primer 69wa: 5'-CGCCATTTCTGGATAATTCTGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11