Genotyping and primer details


Genotyping and primer details for 263D05 - At5g62050
Line ID 263D05
Locus At5g62050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24924764 of line 263D05 Chr5:24924764
   
Insertion allele amplicon 8474 - PH52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH52: 5'-GGAAGTCATCCTCACTTTTGCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24925483
   
wt amplicon (over InsPos) 11fi - PH52
wt amplicon start Chr5:24924286
wt amplicon end Chr5:24925483
wt amplicon size 1198 bp
Locus specific primer PH52: 5'-GGAAGTCATCCTCACTTTTGCTAC-3'
2nd locus specific primer 11fi: 5'-CAGGTATGCGTAACATCTTCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11