Genotyping and primer details


Genotyping and primer details for 265D09 - At5g19580
Line ID 265D09
Locus At5g19580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6608537 of line 265D09 Chr5:6608537
   
Insertion allele amplicon 8474 - 14kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14kh: 5'-ACGTTTAGCTCCGTTGACTAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6608189
   
wt amplicon (over InsPos) 12ky - 14kh
wt amplicon start Chr5:6608189
wt amplicon end Chr5:6609072
wt amplicon size 884 bp
Locus specific primer 14kh: 5'-ACGTTTAGCTCCGTTGACTAGAAG-3'
2nd locus specific primer 12ky: 5'-TTAAGCCTCTAGGGGTATGTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11